Evaluation of synthetic peptides of WbSXP-1 for the diagnosis of human lymphatic filariasis.
Identifieur interne : 005911 ( Main/Exploration ); précédent : 005910; suivant : 005912Evaluation of synthetic peptides of WbSXP-1 for the diagnosis of human lymphatic filariasis.
Auteurs : Pandurangan Pandiaraja [Inde] ; Chakkaravarthy Arunkumar ; Subhash Laxmanappa Hoti ; Donthamsetty Nageswara Rao ; Perumal KalirajSource :
- Diagnostic microbiology and infectious disease [ 1879-0070 ] ; 2010.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antihelminthe (sang), Antigènes d'helminthe (), Antigènes d'helminthe (génétique), Antigènes d'helminthe (immunologie), Antigènes d'helminthe (métabolisme), Données de séquences moléculaires, Filariose lymphatique (diagnostic), Filariose lymphatique (immunologie), Filariose lymphatique (parasitologie), Humains, Peptides (), Peptides (immunologie), Peptides (synthèse chimique), Protéines recombinantes (génétique), Protéines recombinantes (immunologie), Protéines recombinantes (métabolisme), Séquence d'acides aminés, Test ELISA (), Wuchereria bancrofti (immunologie), Épitopes immunodominants (), Épitopes immunodominants (génétique), Épitopes immunodominants (immunologie).
- MESH :
- diagnostic : Filariose lymphatique.
- génétique : Antigènes d'helminthe, Protéines recombinantes, Épitopes immunodominants.
- immunologie : Antigènes d'helminthe, Filariose lymphatique, Peptides, Protéines recombinantes, Wuchereria bancrofti, Épitopes immunodominants.
- métabolisme : Antigènes d'helminthe, Protéines recombinantes.
- parasitologie : Filariose lymphatique.
- sang : Anticorps antihelminthe.
- synthèse chimique : Peptides.
- Animaux, Antigènes d'helminthe, Données de séquences moléculaires, Humains, Peptides, Séquence d'acides aminés, Test ELISA, Épitopes immunodominants.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Helminth (blood), Antigens, Helminth (chemistry), Antigens, Helminth (genetics), Antigens, Helminth (immunology), Antigens, Helminth (metabolism), Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (immunology), Elephantiasis, Filarial (parasitology), Enzyme-Linked Immunosorbent Assay (methods), Humans, Immunodominant Epitopes (chemistry), Immunodominant Epitopes (genetics), Immunodominant Epitopes (immunology), Molecular Sequence Data, Peptides (chemical synthesis), Peptides (chemistry), Peptides (immunology), Recombinant Proteins (genetics), Recombinant Proteins (immunology), Recombinant Proteins (metabolism), Wuchereria bancrofti (immunology).
- MESH :
- chemical , blood : Antibodies, Helminth.
- chemical , chemical synthesis : Peptides.
- chemical , chemistry : Antigens, Helminth, Immunodominant Epitopes, Peptides.
- chemical , genetics : Antigens, Helminth, Immunodominant Epitopes, Recombinant Proteins.
- chemical , immunology : Antigens, Helminth, Immunodominant Epitopes, Peptides, Recombinant Proteins.
- chemical , metabolism : Antigens, Helminth, Recombinant Proteins.
- diagnosis : Elephantiasis, Filarial.
- immunology : Elephantiasis, Filarial, Wuchereria bancrofti.
- methods : Enzyme-Linked Immunosorbent Assay.
- parasitology : Elephantiasis, Filarial.
- Amino Acid Sequence, Animals, Humans, Molecular Sequence Data.
Abstract
Parasitic nematodes infect nearly half of the world's human population, resulting in significant morbidity and mortality. Though filariasis is not fatal, it is the second leading cause of permanent and long-term disability worldwide. Filariasis has a spectrum of disease manifestation and infectivity found among the infected individuals and also goes unnoticed for years. Furthermore, there are ample reports emerging on the genetic variation among the parasites population. Hence, it is necessary to develop diagnostics for early detection of the disease. Synthetic peptides that mimic the immunogenic regions and a conserved region similar to that of recombinant antigen will be more useful in developing diagnostics, vaccines, or therapeutics. WbSXP-1 was earlier proven as a good diagnostic antigen; B-cell epitopic analysis showed 4 potent immunodominant regions spanning the whole antigen. These synthetic peptides (N, N1, N2, and N3) were produced and used as a diagnostic candidate to detect anti-SXP antibody and conversely to detect the infected individuals. The monomeric peptides showed good reactivity against microfilareamic (MF) sera. Among them, the peptides N, N1, and N2 were found to be more reactive. Furthermore, multiple chimeric peptides in linear combinations of 2 peptides were tested for its efficacy to detect anti-SXP antibody in infected MF sera. The peptides N:N1 and N1:N2 were synthesized and tested against human clinical sera. This chimeric peptides constructed based on WbSXP-1 were found to be reactive, specifically with MF sera by ELISA. These peptide-based diagnostic method can serve as a standard better tool without cross-reactivity in lymphatic filariasis elimination program.
DOI: 10.1016/j.diagmicrobio.2010.07.015
PubMed: 21094425
Affiliations:
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Le document en format XML
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(immunology)</term><term>Recombinant Proteins (metabolism)</term><term>Wuchereria bancrofti (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antihelminthe (sang)</term><term>Antigènes d'helminthe ()</term><term>Antigènes d'helminthe (génétique)</term><term>Antigènes d'helminthe (immunologie)</term><term>Antigènes d'helminthe (métabolisme)</term><term>Données de séquences moléculaires</term><term>Filariose lymphatique (diagnostic)</term><term>Filariose lymphatique (immunologie)</term><term>Filariose lymphatique (parasitologie)</term><term>Humains</term><term>Peptides ()</term><term>Peptides (immunologie)</term><term>Peptides (synthèse chimique)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (immunologie)</term><term>Protéines recombinantes (métabolisme)</term><term>Séquence d'acides aminés</term><term>Test ELISA ()</term><term>Wuchereria bancrofti (immunologie)</term><term>Épitopes 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Immunosorbent Assay</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antigènes d'helminthe</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antihelminthe</term></keywords><keywords scheme="MESH" qualifier="synthèse chimique" xml:lang="fr"><term>Peptides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Humans</term><term>Molecular Sequence Data</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Antigènes d'helminthe</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Peptides</term><term>Séquence d'acides aminés</term><term>Test ELISA</term><term>Épitopes immunodominants</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Parasitic nematodes infect nearly half of the world's human population, resulting in significant morbidity and mortality. Though filariasis is not fatal, it is the second leading cause of permanent and long-term disability worldwide. Filariasis has a spectrum of disease manifestation and infectivity found among the infected individuals and also goes unnoticed for years. Furthermore, there are ample reports emerging on the genetic variation among the parasites population. Hence, it is necessary to develop diagnostics for early detection of the disease. Synthetic peptides that mimic the immunogenic regions and a conserved region similar to that of recombinant antigen will be more useful in developing diagnostics, vaccines, or therapeutics. WbSXP-1 was earlier proven as a good diagnostic antigen; B-cell epitopic analysis showed 4 potent immunodominant regions spanning the whole antigen. These synthetic peptides (N, N1, N2, and N3) were produced and used as a diagnostic candidate to detect anti-SXP antibody and conversely to detect the infected individuals. The monomeric peptides showed good reactivity against microfilareamic (MF) sera. Among them, the peptides N, N1, and N2 were found to be more reactive. Furthermore, multiple chimeric peptides in linear combinations of 2 peptides were tested for its efficacy to detect anti-SXP antibody in infected MF sera. The peptides N:N1 and N1:N2 were synthesized and tested against human clinical sera. This chimeric peptides constructed based on WbSXP-1 were found to be reactive, specifically with MF sera by ELISA. These peptide-based diagnostic method can serve as a standard better tool without cross-reactivity in lymphatic filariasis elimination program.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Arunkumar, Chakkaravarthy" sort="Arunkumar, Chakkaravarthy" uniqKey="Arunkumar C" first="Chakkaravarthy" last="Arunkumar">Chakkaravarthy Arunkumar</name><name sortKey="Hoti, Subhash Laxmanappa" sort="Hoti, Subhash Laxmanappa" uniqKey="Hoti S" first="Subhash Laxmanappa" last="Hoti">Subhash Laxmanappa Hoti</name><name sortKey="Kaliraj, Perumal" sort="Kaliraj, Perumal" uniqKey="Kaliraj P" first="Perumal" last="Kaliraj">Perumal Kaliraj</name><name sortKey="Rao, Donthamsetty Nageswara" sort="Rao, Donthamsetty Nageswara" uniqKey="Rao D" first="Donthamsetty Nageswara" last="Rao">Donthamsetty Nageswara Rao</name></noCountry><country name="Inde"><noRegion><name sortKey="Pandiaraja, Pandurangan" sort="Pandiaraja, Pandurangan" uniqKey="Pandiaraja P" first="Pandurangan" last="Pandiaraja">Pandurangan Pandiaraja</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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